Olivia King

• Phone: +1 416 555 0123
• Email: olivia.king@email.com
• Location: Toronto, Canada
• LinkedIn: oliviakingbiophysicist

Summary

Six years of experience in biophysical characterization of macromolecules, focusing on protein-ligand interactions and structural biology. Led projects to elucidate mechanisms of action for novel therapeutic compounds, contributing to pipeline advancement in pharmaceutical research. Proficient in advanced spectroscopic techniques and computational modeling for drug design and optimization.

Experience

Senior Biophysicist, Veridian Pharmaceuticals Inc. -- Toronto, Canada

Mar 2021 – present

• Spearheaded biophysical characterization of 15+ small molecule drug candidates, providing critical data for lead optimization and selection.

• Implemented and optimized Surface Plasmon Resonance (SPR) workflows, increasing throughput by 30% for binding kinetics analysis.

• Utilized Differential Scanning Fluorimetry (DSF) and Isothermal Titration Calorimetry (ITC) to assess protein stability and thermodynamic binding profiles for 8 oncology targets.

• Mentored junior scientists in experimental design, data analysis, and interpretation of biophysical data.

Research Biochemist, Aurora Biotech Solutions -- Toronto, Canada

July 2018 – Feb 2021

• Conducted extensive biophysical studies on membrane proteins using Circular Dichroism (CD) and Dynamic Light Scattering (DLS) to understand conformational changes.

• Developed and validated fluorescence-based assays for high-throughput screening of enzyme inhibitors, identifying 5 novel hit compounds.

• Collaborated with structural biology teams to integrate biophysical data with X-ray crystallography and cryo-EM structures, enhancing mechanistic understanding.

• Authored 3 peer-reviewed publications detailing findings on protein-protein interaction interfaces.

Education

University of Toronto, Ph.D. in Biochemistry and Biophysics -- Toronto, Canada

Sept 2013 – June 2018

McGill University, B.Sc. (Honours) in Biochemistry -- Montreal, Canada

Sept 2009 – May 2013

Skills

Biophysical Techniques: Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), Differential Scanning Fluorimetry (DSF), Circular Dichroism (CD), Dynamic Light Scattering (DLS), Fluorescence Spectroscopy, Mass Spectrometry

Molecular Biology & Biochemistry: Protein Expression & Purification, Enzyme Kinetics, Ligand Binding Assays, ELISA, Western Blotting, SDS-PAGE, PCR, CRISPR/Cas9

Data Analysis & Software: GraphPad Prism, OriginPro, KaleidaGraph, Scrubber, BioRender, PyMOL, ChemDraw, Microsoft Office Suite

Computational Tools: Molecular Docking, Molecular Dynamics Simulation (basic), Bioinformatics Databases (UniProt, PDB)

Laboratory Management: SOP Development, Equipment Maintenance, GLP Compliance, Project Management, Team Leadership